Evaluation of a Modification of the Zak-kiliani Reaction for Determination of Serum Cholesterol.

A rapid and specific ultramicro method for total serum cholesterol.

A modification of the Zak cholesterol reaction is described in which ethanol is substituted for glacial acetic acid. This modification avoids the hazard of purifying glacial acetic acid and also increases the specificity of the reaction by eliminating ir.terference from bromide or iodide salts. The ethanolic reaction has the absorbance spectrum, sensitivity, and stability of the usual acetic ac...

A simple method for the determination of serum cholesterol

CholesterolMethodologies : A Review Bennie Zak

The introduction of enzymes as reagents into the methodologies of cholesterol determination (1-6) has drastically altered the limited strong-acid approaches taken by most analysts of the past 90 years (7-19). There has been a considerably heightened interest in determining the compound because of new diagnostic approaches such as hyperlipoprotein phenotyping (13) with the implied relationship o...

The determination of cholesterol and coprosterol in faecal lipids.

Snog-Kjaer, Prange & Dam (1956) and Coleman & Baumann (1957) have demonstrated the microbial conversion of cholesterol into coprosterol in the mammalian large intestine. Thus there is need for the rapid and accurate simultaneous quantitative determination of cholesterol and coprosterol. This is attempted in the chromatographic procedure of Coleman, Wells & Baumann (1956), which is too time-cons...

مقایسه اثرات سبوس برنج و سبوس گندم بر فراسنج های لیپیدی سرم در زنان هیپرکلسترولمیک

Background: Cardiovascular diseases are the most important causes of mortality in Iran. Hypercholesterolemia is one of the major risk factors for cardiovascular diseases. Dietary modification such as cereal bran consumption is an approach to reduce this risk factor. The purpose of this present study was to compare the effects of rice bran and wheat bran on serum lipids in hypercholesterolemic w...